micro-propagation workshop

hostajim1 Port Orchard, WA(Zone 8a) Oct 30, 2005

I went to micro-prop workshop Oct. 29 for the hobbyist. I'm definetely going to pursue this with hosta as my main subject. now that I can do it for less than $100 verses thousands for a laboratory. I was wondering when it would come down to the home, hobby level, good luck to anyone interested in this. hostajim1

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growin Beautiful, BC(Zone 8b) Oct 30, 2005

Hi hostajim1, any tips, trick, comments you can help me with would be greatly appreciated. I'm still trying to find the equipment for cheap. Thanks!

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hostajim1 Port Orchard, WA(Zone 8a) Oct 31, 2005

growin. I just made a clean box for transfering tissue to media boxes, $10 in material. now I'm looking for a supply of baby food jars. (day Care Centers ) 6oz jars a great for this. I'm looking for wire racks for layering worklights and media jars. Carol of Kitchen Culture Kits has all this info if you check it out.

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growin Beautiful, BC(Zone 8b) Nov 03, 2005

hostajim1, I thought you might like this link: http://www.hostatissueculture.com/

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hostajim1 Port Orchard, WA(Zone 8a) Nov 03, 2005

growin, I put the site on my favorites list, thanks my TC lab is coming together, I'm making my own plant grow shelves with worklights. a lot cheaper $125 verses $500 to $900 in greenhouse catalog. I'm getting close to starting.

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growin Beautiful, BC(Zone 8b) Nov 04, 2005

Hi Hostajim1, I also found a number of local sources for materials yesterday so I'm getting there. I also found a few links that may be of some help.
http://groups.yahoo.com/group/hometissueculture/ (Yahoo Tissue Culture Group)
http://www.omnisterra.com/botany/cp/slides/tc/tc.htm (Carnivorous Plants)
I found this last link to be the most help with simple pics and instructions. The use of a side-turned aquarium seems so easy & practical for a hood. On the Hosta site, under http://www.hostatissueculture.com/gettingbiggerfaster.htm
is an interesting article about BAP-10 which significantly increases offsets on inground plants.
Cheers

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Botanyguy Titusville, FL(Zone 9a) Jan 01, 2006

Jim I too use micropropagation for hostas, if you can shoot me over na email and we can compare notes. I have a whole room dedicated to a lab at my home. I have had some great results from "over soaking" hosta seeds in bleacl solution until the seed outer coating is soaked off (I use a magnetic stirring system), I then put my seeds in 1/5 MS after a triple rise. I get approx a 99% germination compared to not stripping the outer coat at an approx 18% germination rate, this is based on a dual batch of 300 seeds each.

Chris